Studies on a new oxalate-decomposing bacterium, Pseudomonas oxalaticus.

Energy Production and Growth of Pseudomonas oxalaticus OXl on Oxalate and Formate

The efficiency of oxidative phosphorylation in Pseudomonas oxalaticus during growth on oxalate and formate was estimated by two methods. In the first method the amount of ATP required to synthesize cell material of standard composition was calculated during growth of the organism on either of the two substrates. The [Y2~] theor, values thus obtained were 12,5 and 6.5 for oxalate and formate res...

Energy Production and Growth of Pseudomonas oxalaticus OXl on Oxalate and Formate

The efficiency of oxidative phosphorylation in Pseudomonas oxalaticus during growth on oxalate and formate was estimated by two methods. In the first method the amount of ATP required to synthesize cell material of standard composition was calculated during growth of the organism on either of the two substrates. The [Y2~] theor, values thus obtained were 12,5 and 6.5 for oxalate and formate res...

Carbon assimilation by Pseudomonas oxalaticus (OXI). 4. Metabolism of oxalate in cell-free extracts of the organism grown on oxalate.

The oxidation level of oxalate is such that incorporation of its carbon into cell constituents must involve a net reduction. If it is the sole carbon source for microbial growth then it must also undergo condensation reactions resulting in the synthesis of, for example, C3 and C4 units necessary for formation of carbohydrate and amino acid carbon skeletons (for a review of this aspect, see Korn...

Use of an isothermal microcalorimetry assay to characterize microbial oxalotrophic activity.

Isothermal microcalorimetry (IMC) has been used in the past to monitor metabolic activities in living systems. A few studies have used it on ecological research. In this study, IMC was used to monitor oxalotrophic activity, a widespread bacterial metabolism found in the environment, and particularly in soils. Six model strains were inoculated in solid angle media with K-oxalate as the sole carb...

University of Groningen Regulation of Autotrophic and Heterotrophic Metabolism in Pseudomonas oxalaticus OX1. Growth on Fructose and on Mixtures of Fructose and Formate in Batch and Continuous Cultures